RNA
Part:BBa_M36132:Design
Designed by: Vir Choksi, Daniel McHugh Group: Stanford BIOE44 - S11 (2012-06-01)
2D inducible RNA scaffold
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 71
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 71
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 71
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 71
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.
Source
RNA scaffold comes from Delebecque et al., Science 2011 Ribozyme switch comes from Win & Smolke, PNAS 2007