RNA

Part:BBa_M36132:Design

Designed by: Vir Choksi, Daniel McHugh   Group: Stanford BIOE44 - S11   (2012-06-01)


2D inducible RNA scaffold


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.


Source

RNA scaffold comes from Delebecque et al., Science 2011 Ribozyme switch comes from Win & Smolke, PNAS 2007

References